Blar i forfatter "Singh, Bhupender"

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    • Drug-target binding quantitatively predicts optimal antibiotic dose levels in quinolones 

      Clarelli, Fabrizio; Palmer, Adam; Singh, Bhupender; Storflor, Merete; Lauksund, Silje; Cohen, Ted; Abel, Sören; Abel zur Wiesch, Pia (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-14)
      Antibiotic resistance is rising and we urgently need to gain a better quantitative understanding of how antibiotics act, which in turn would also speed up the development of new antibiotics. Here, we describe a computational model (COMBAT-COmputational Model of Bacterial Antibiotic Target-binding) that can quantitatively predict antibiotic dose-response relationships. Our goal is dual: We address a ...

    • Flagella-mediated secretion of a novel Vibrio cholerae cytotoxin affecting both vertebrate and invertebrate hosts 

      Dongre, Mitesh; Singh, Bhupender; Aung, Kyaw Min; Larsson, Per; Miftakhova, Regina; Persson, Karina; Askarian, Fatemeh; Johannessen, Mona; Hofsten, Jonas von; Persson, Jenny L.; Erhardt, Marc; Tuck, Simon; Uhlin, Bernt Eric; Wai, Sun Nyunt (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-06-07)
      Using <i>Caenorhabditis elegans</i> as an infection host model for <i>Vibrio cholerae</i> predator interactions, we discovered a bacterial cytotoxin, MakA, whose function as a virulence factor relies on secretion via the flagellum channel in a proton motive force-dependent manner. The MakA protein is expressed from the polycistronic <i>makDCBA</i> (<i>m</i>otility-<i>a</i>ssociated <i>k</i>illing ...

    • Multifunctional Nanofibrous Dressing with Antimicrobial and Anti-Inflammatory Properties Prepared by Needle-Free Electrospinning 

      Schulte Werning, Laura; Murugaiah, Anjanah; Singh, Bhupender; Johannessen, Mona; Engstad, Rolf Einar; Skalko-Basnet, Natasa; Holsæter, Ann Mari (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-09-21)
      An active wound dressing should address the main goals in wound treatment, which are improved wound healing and reduced infection rates. We developed novel multifunctional nanofibrous wound dressings with three active ingredients: chloramphenicol (CAM), beta-glucan (βG) and chitosan (CHI), of which βG and CHI are active nanofiber-forming biopolymers isolated from the cell walls of Saccharomyces ...

    • Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo 

      Joshi, Bishnu; Singh, Bhupender; Nadeem, Aftab; Askarian, Fatemeh; Wai, Sun Nyunt; Johannessen, Mona; Hegstad, Kristin (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-13)
      Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of ...